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Updated: Jul 9, 2026
04:19
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
Published on: April 19, 2019
Summary
A new surgical technique allows subcutaneous spleen transposition in rats for reliable transplenic portal injections. This method aids in studying portal circulation, liver function, and drug actions.
Area of Science:
- Veterinary surgery
- Surgical techniques
- Animal models
Background:
- The portal circulation is crucial for liver function and drug metabolism.
- Investigating portal hemodynamics and liver function requires accessible and reliable animal models.
- Current methods for studying portal circulation in rats have limitations.
Purpose of the Study:
- To describe a novel technique for subcutaneous spleen transposition in rats.
- To establish a reliable method for transplenic portal injection.
- To evaluate the utility of this technique for studying portal circulation and liver function.
Main Methods:
- Subcutaneous transposition of the spleen in rats.
- Transplenic injection of various substances into the spleen pulp.
- Radiological and isotope-based detection of porta-systemic venous communications.
- Ligation of the portal vein above the splenic tributary.
Main Results:
- The transposed spleen allows for reliable transplenic portal injections.
- Substances injected into the spleen pulp are cleared, not pooled.
- Development of porta-systemic venous communications was demonstrated.
- Animals survived portal vein ligation if adequate porta-systemic connections were present.
Conclusions:
- Subcutaneous spleen transposition in rats provides a reliable method for transplenic portal injection.
- This technique facilitates the study of portal circulation, liver function, and drug action.
- The method offers a simple approach for inducing porta-systemic diversion in rats.
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