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Modification of the microtiter reading mirror for use in the standardized micro complement fixation test
Applied Microbiology
|June 1, 1971
Abstract:
Modification of the Microtiter reading mirror used in the standardized diagnostic complement fixation method permits convenient estimation of the results in per cent hemolysis by direct visual comparison with the hemolytic standards.
Insights
A modified Microtiter reading mirror allows easy visual estimation of results in the complement fixation test. This method simplifies quantifying percent hemolysis by comparing directly against hemolytic standards.
Area of Science:
- Immunology
- Diagnostic assay development
Background:
- The complement fixation test is a standard diagnostic method.
- Accurate quantification of hemolysis is crucial for test interpretation.
Purpose of the Study:
- To develop a convenient method for estimating results in the complement fixation test.
- To enable direct visual comparison of hemolysis with standards.
Main Methods:
- Modification of the Microtiter reading mirror.
- Standardized diagnostic complement fixation method.
- Visual comparison with hemolytic standards.
Main Results:
- The modified mirror allows convenient estimation of results.
- Results can be expressed as percent hemolysis.
- Direct visual comparison is feasible.
Conclusions:
- The modified Microtiter reading mirror simplifies complement fixation test result interpretation.
- This modification offers a practical approach for quantifying hemolysis.