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"Upper limits of normal" antistreptolysin O and antideoxyribonuclease B titers
Insights
Normal antibody titers for antistreptolysin O (ASO) and antideoxyribonuclease B (ADN-B) vary significantly by age. These upper limits of normal are crucial for accurately diagnosing recent streptococcal infections in children and adults.
Area of Science:
- Pediatric Immunology
- Clinical Chemistry
- Microbiology
Background:
- Accurate interpretation of antistreptolysin O (ASO) and antideoxyribonuclease B (ADN-B) antibody titers is essential for diagnosing streptococcal infections.
- Establishing age-specific
- upper limits of normal
- is critical for accurate diagnostic reference ranges.
Purpose of the Study:
- To determine the
- upper limits of normal
- for ASO and ADN-B titers across different pediatric age groups and healthy adults.
- To identify age-related variations in these antibody titers.
Main Methods:
- Serum specimens were collected from pediatric patients without recent streptococcal infection history and healthy adult hospital employees.
- Antibody titers (ASO and ADN-B) were measured.
- The
- upper limit of normal
- was defined as the titer exceeded by no more than 15% of subjects within each age group.
Main Results:
- Significant age-dependent variations were observed in both ASO and ADN-B titers.
- Preschool children showed lower upper limits (ASO: 85, ADN-B: 60) compared to school-aged children (ASO: 170, ADN-B: 170).
- Adults exhibited upper limits similar to preschool children (ASO: 85, ADN-B: 85).
Conclusions:
- The
- upper limits of normal
- for ASO and ADN-B titers are age-specific, with notable differences between preschool, school-aged children, and adults.
- These findings necessitate the use of age-adjusted reference ranges for accurate interpretation of streptococcal antibody titers.
- Establishing these age-specific norms aids in the precise diagnosis of streptococcal sequelae.
Abstract:
"Upper limits of normal" antistreptolysin O (ASO) and antideoxyribonuclease (ADN) B titers were determined on serum specimens from various age groups of pediatric patients with no history of a recent streptococcal infection and on healthy adult hospital employees. The upper limit of normal value is that level of antibody titer exceeded by no more than 15% of the total subjects in each age group. This value varies with the age of the subject, and the most pronounced differences are between the values of preschool age children, school age children, and adults. The upper limit of normal values for these groups were as follows: preschool age, ASO = 85(100); ADN B = 60(50); school age, ASO = 170(166); ADN B=1 70(166); and adult, ASO = 85(100); ADN B = 85(100).