Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A radioimmunoassay for human serum myoglobin: method development and normal values.

T G Rosano, M A Kenny

    Clinical Chemistry
    |January 1, 1977
    PubMed
    Summary

    A new radioimmunoassay accurately measures serum myoglobin, aiding in the assessment of muscle mass and related conditions. This method shows negligible cross-reactivity with hemoglobin.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Should laparoscopic surgeons wear masks?

    The Journal of hospital infection·2009
    Same author

    Treatment-resistant depressed patients show a good response to Mindfulness-based Cognitive Therapy.

    Behaviour research and therapy·2006
    Same author

    Fatal strychnine poisoning: application of gas chromatography and tandem mass spectrometry.

    Journal of analytical toxicology·2000
    Same author

    Urinary free deoxypyridinoline by chemiluminescence immunoassay: analytical and clinical evaluation.

    Clinical chemistry·1998
    Same author

    Use of pharmacokinetics to determine the duration of dialysis in management of methanol poisoning.

    The American journal of emergency medicine·1998
    Same author

    Feasibility study of the spectroscopic measurement of oxyhemoglobin using whole blood without pre-treatment.

    The Analyst·1998

    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • Myoglobin is a protein found in muscle tissue.
    • Accurate measurement of serum myoglobin is important for clinical diagnostics.
    • Existing methods may have limitations in sensitivity or specificity.

    Purpose of the Study:

    • To develop and validate a radioimmunoassay (RIA) for quantifying serum myoglobin.
    • To assess the assay's performance characteristics, including sensitivity, precision, and specificity.
    • To explore the relationship between serum myoglobin levels and muscle mass.

    Main Methods:

    • Purification of human skeletal muscle myoglobin.
    • Production of rabbit antisera against purified myoglobin.
    • Radiolabeling of myoglobin using [125]-labeled N-hydroxysuccinimide ester.
    • Development of a RIA protocol with simultaneous reagent addition and a 3.5-hour incubation at 37°C.
    • Separation of antibody-bound myoglobin via polyethylene glycol precipitation.

    Main Results:

    • The developed RIA can measure both normal and elevated serum myoglobin concentrations.
    • The assay demonstrates good precision, linearity, and recovery.
    • Negligible cross-reactivity with human hemoglobin was observed.
    • Information on detection limits and specimen preservation is provided.

    Conclusions:

    • A robust and specific radioimmunoassay for serum myoglobin has been established.
    • This assay is suitable for measuring myoglobin levels in clinical settings.
    • Further investigation into the correlation between serum myoglobin and muscle mass is warranted.

    Related Experiment Videos