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Decrease in nitrate reductase activity in extracts of Trichoderma viride Incubated with chlorides.

W L Balderston, J J Rowe, W J Payne

    Journal of Bacteriology
    |March 1, 1977
    PubMed
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    Ammonium chloride, sodium chloride, and potassium chloride inhibit Trichoderma virde nitrate reductase activity. This chloride inhibition of nitrate reductase activity increases linearly with chloride concentration.

    Area of Science:

    • Biochemistry
    • Mycology
    • Enzymology

    Background:

    • Nitrate reductase is a key enzyme in nitrogen assimilation in fungi.
    • Trichoderma virde is a common soil fungus with implications in agriculture.
    • Understanding enzyme regulation is crucial for fungal physiology.

    Purpose of the Study:

    • To investigate the effect of various salts on nitrate reductase activity in Trichoderma virde.
    • To determine the specific ions responsible for inhibition.
    • To characterize the dose-dependent relationship of chloride inhibition.

    Main Methods:

    • Crude enzyme extracts of Trichoderma virde were prepared.
    • Enzyme activity assays were performed in the presence of different salt concentrations.
    • Spectrophotometric methods were used to measure nitrate reductase activity.

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    Main Results:

    • Ammonium chloride, sodium chloride, and potassium chloride significantly inhibited nitrate reductase activity.
    • Ammonium sulfate and sodium sulfate did not show significant inhibition.
    • Chloride inhibition demonstrated a linear correlation with increasing chloride concentrations.

    Conclusions:

    • Chloride ions are potent inhibitors of Trichoderma virde nitrate reductase.
    • The findings suggest a specific inhibitory mechanism by chloride ions.
    • This has implications for understanding nitrogen metabolism in Trichoderma under saline conditions.