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Oncogenicity of a nude mouse cell line transformed by a human papovavirus
Abstract:
Primary cultures of NIH nude mouse (nu/nu) kidney cells were transformed with a human papovavirus (MMV). The transformed cell line expressed T-antigen, and MMV DNA was found to be associated with the cell DNA. When NIH nu/nu mice were inoculated with the transformed cells, they developed tumors at the injection site but failed to generate detectable levels of T-antibody. A control group of nu/+ littermates rejected the tumor inoculum but mounted an antibody response to T-antigen. It was proposed that nude mouse cells may be a suitable system to test oncogenicity of in vitro transformed cells.
Insights
NIH nude mouse kidney cells transformed with a human papovavirus (MMV) developed tumors in nude mice. Control mice rejected tumors and showed an antibody response, suggesting nude mouse cells can test oncogenicity.
Area of Science:
- Oncology
- Virology
- Immunology
Background:
- Nude mice (nu/nu) are immunodeficient, impacting their ability to reject foreign cells.
- Human papovavirus (MMV) can transform cells and potentially cause tumors.
Purpose of the Study:
- To investigate the oncogenic potential of MMV-transformed NIH nude mouse kidney cells.
- To evaluate the immune response of nude mice and their littermates to these transformed cells.
Main Methods:
- Primary NIH nude mouse kidney cells were transformed in vitro using MMV.
- Transformed cells expressing T-antigen and MMV DNA were inoculated into nude mice (nu/nu) and control littermates (nu/+).
- Tumor development and T-antibody responses were monitored in both groups.
Main Results:
- NIH nu/nu mice inoculated with transformed cells developed tumors at the injection site.
- These nude mice failed to produce detectable T-antibody levels.
- Control nu/+ littermates rejected the tumor inoculum and mounted an antibody response to T-antigen.
Conclusions:
- NIH nude mouse cells transformed with MMV can induce tumors in nude mice.
- Nude mice exhibit a deficient antibody response to T-antigen in this model.
- Nude mouse cells offer a potential system for testing the oncogenicity of in vitro transformed cells.