[Production of pullulan]

Optimum conditions for the production of pullulan by Pullularia pullulans were elaborated by examinating growth and production with different carbohydrates and mixtures of carbohydrates. With starch hydrolysates or mixtures of starch and simple sugars, p.e. glucose or saccharose, better results were achieved than with simple sugars alone or with starch. Condtions for optimum pullulan production are fast undisturbed growth, mediated by fast utilizable simple sugars like glucose followed by a substantial decreased growth rate in which production occurs, mediated by a sufficient supply of nutrite substances and a slow utilizable carbohydrate like starch.