[Immunogenic properties of DNA from E. coli and T4 phage, containing 5-bromouracil]

5-BUDR incorporated into E. coli or T4 phage DNAs is an immunogenic determinant. Immunodominance of 5-BUDR is determined by the chemical structure of DNA molecule into which it is incorporated. In E. coli DNA, 5-BUDR inhibits immunogenic activity of all other bases. One can conclude that in this DNA 5-BUDR is the dominant antigenic determinant. In T4 DNA, 5-BUDR acts as an additional antigenic determinant. Anti-5-BUDR-T4-DNA contains antibodies to 5-BUDR and glucosylated 5-HMC.