Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method of applying the Sips equation.

M H Nahm, L A Herzenberg, K Little

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1977
    PubMed
    Summary

    A new method simplifies determining antibody-binding characteristics using the Sips equation. It calculates affinity and concentration without needing prior antibody concentration or full ligand saturation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Program logic of a mental health outreach service for socially anxious youth.

    Evaluation and program planning·2023
    Same author

    Oncoplastic breast surgery: A guide to good practice.

    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2021
    Same author

    Life expectancy inequalities in Wales before COVID-19: an exploration of current contributions by age and cause of death and changes between 2002 and 2018.

    Public health·2021
    Same author

    Collaborative meta-analysis finds no evidence of a strong interaction between stress and 5-HTTLPR genotype contributing to the development of depression.

    Molecular psychiatry·2017
    Same author

    Parent insights into atypicalities of social approach behaviour in Williams syndrome.

    Journal of intellectual disability research : JIDR·2016
    Same author

    Serum Beta Hydroxybutyrate Concentrations in Cats with Chronic Kidney Disease, Hyperthyroidism, or Hepatic Lipidosis.

    Journal of veterinary internal medicine·2016

    Area of Science:

    • Immunochemistry
    • Biophysical Chemistry
    • Chemical Kinetics

    Background:

    • The Sips equation is standard for analyzing antibody-ligand interactions, relating antibody concentration, affinity, and heterogeneity.
    • Current methods typically require pre-determining antibody-binding site concentration (n0[Ab]) and extensive calculations for affinity (K) and heterogeneity (a).

    Purpose of the Study:

    • To introduce a novel method for determining Sips equation parameters (K and a) without prior knowledge of n0[Ab].
    • To enable determination of antibody-binding site concentration without purified antibodies or ligand saturation.
    • To provide a generalized approach applicable to various antibody-monovalent ligand systems and ordinary chemical reactions.

    Main Methods:

    • A new mathematical approach to solve the Sips equation without pre-determined antibody concentration.
    • Utilizes the relationship between antibody-binding site concentration, antigen concentration, intrinsic affinity, and heterogeneity index.
    • Applies to systems where antibody concentration exceeds 1/K.

    Main Results:

    • Successfully determines affinity constant (K) and heterogeneity index (a) without needing initial antibody concentration (n0[Ab]).
    • Enables calculation of n0[Ab] without purified antibody or achieving ligand saturation.
    • Demonstrates applicability to monovalent ligand binding and general chemical reactions (by setting a=1).

    Conclusions:

    • The proposed method offers a simplified and versatile approach to characterizing antibody-ligand interactions.
    • It reduces experimental requirements, such as purified antibody or ligand saturation.
    • This method enhances the general applicability of the Sips equation in chemical and biological binding studies.

    Related Experiment Videos