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Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
Published on: May 30, 2013
Hog cholera virus : sensitivity to hydrolytic enzymes
Annales De Recherches Veterinaires. Annals of Veterinary Research
|January 1, 1977
Summary
Hog Cholera virus (HCV) infectivity significantly decreases with trypsin and phospholipase C, indicating its reliance on membrane phospholipids. This sensitivity explains field behavior but not strain pathogenicity.
Area of Science:
- Virology
- Biochemistry
Background:
- Hog Cholera virus (HCV) is an economically significant swine pathogen.
- Understanding viral structure and its interaction with enzymes is crucial for disease control.
Purpose of the Study:
- To investigate the effect of trypsin and phospholipase C on Hog Cholera virus infectivity.
- To elucidate the role of membrane phospholipids in HCV infectivity and behavior.
Main Methods:
- Enzyme treatment: Exposure of HCV to trypsin and phospholipase C.
- Infectivity assays: Quantifying viral infectivity after enzyme treatment.
- Kinetic analysis: Determining the rate of viral inactivation.
Main Results:
- Both trypsin and phospholipase C markedly reduced HCV infectivity.
- Viral inactivation kinetics demonstrated a significant decrease in infectivity upon enzyme exposure.
- No correlation was found between inactivation rate and the pathogenicity of different HCV strains.
Conclusions:
- Hog Cholera virus infectivity is dependent on the integrity of its membrane phospholipids.
- The virus's sensitivity to proteolytic action provides insights into its field behavior.
- Enzyme sensitivity does not correlate with viral strain pathogenicity.
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