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Biological and biophysical characteristics of mouse adenovirus, strain FL
Abstract:
Mouse adenovirus, strain FL (MAV), is best propagated in primary rather than secondary cultures of mouse kidney cells. Virus release from the cells into the medium is fairly efficient; the average yield per cell is about 1000 TCID50. Morphological and other biophysical characteristics are those of a typical adenovirus: the icosahedral shell with a diameter of 74 nm, fiber projections of 29 nm length, its localization inside the infected kidney cells, a buoyant density in CsC1 of 1.34 g/ml, inhibition of multiplication by inhibitors of DNA synthesis. The thermostability is higher than that of human adenoviruses, whereas, in contrast to these, MAV is inactivated to a great extent by trypsin. Complete or incomplete hemagglutinin or toxin-like activity were not detected. The virus shows no cross-neutralization with human adenoviruses and a one-side cross-reaction with another murine adenovirus strain K-87. Soluble complement-fixing antigen of MAX exhibits a sedimentation rate of 12S identical to the hexon component of human adenoviruses; both antigens show a partial antigenic relationship in tests with appropriate antisera.
Insights
Mouse adenovirus (MAV) is best grown in primary mouse kidney cells, yielding 1000 TCID50 per cell. This DNA virus shares typical adenovirus characteristics but differs in thermostability and trypsin sensitivity compared to human strains.
Area of Science:
- Virology
- Cell Biology
Background:
- Mouse adenovirus strain FL (MAV) is a significant viral agent in laboratory mice.
- Understanding MAV's propagation and characteristics is crucial for research involving animal models.
Purpose of the Study:
- To characterize the propagation and biophysical properties of Mouse Adenovirus, strain FL (MAV).
- To compare MAV's characteristics with human adenoviruses.
Main Methods:
- Propagation in primary and secondary mouse kidney cell cultures.
- Biophysical characterization including morphology, density, and sedimentation rate.
- Antigenic cross-neutralization tests with human adenoviruses and other murine strains.
Main Results:
- MAV propagates efficiently in primary mouse kidney cells, with yields of approximately 1000 TCID50/cell.
- Biophysical analysis revealed typical adenovirus features (icosahedral shell, density 1.34 g/ml) but higher thermostability and trypsin sensitivity than human adenoviruses.
- No cross-neutralization with human adenoviruses was observed, but a one-way reaction with MAV strain K-87 was noted. The soluble antigen showed partial antigenic relationship with human adenovirus hexon.
Conclusions:
- MAV strain FL exhibits distinct biological and antigenic properties compared to human adenoviruses.
- Primary mouse kidney cell cultures are optimal for MAV propagation.
- MAV shares some antigenic similarities with other murine adenoviruses, suggesting potential for cross-reactivity within the murine adenovirus group.