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Repetitive DNA in differentiating chick tissues.

K N Ayres

    Journal of Cellular Physiology
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Highly repetitive DNA content is consistent across embryonic chick tissues, indicating no specific DNA amplification in somatic cells. This finding applies to cerebrum, muscle, and neural retina at various developmental stages.

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    Area of Science:

    • Developmental Biology
    • Molecular Genetics
    • Genomics

    Background:

    • Investigating DNA amplification in somatic cells is crucial for understanding tissue-specific gene regulation.
    • Repetitive DNA sequences play significant roles in genome structure and function, but their differential amplification in embryonic tissues remains an area of interest.

    Purpose of the Study:

    • To determine if highly repetitive DNA content differs among embryonic chick tissues (cerebrum, muscle, neural retina).
    • To assess whether differential DNA amplification occurs in somatic cells during embryonic development.

    Main Methods:

    • DNA was isolated from embryonic chick cerebrum, muscle, and neural retina at various developmental stages.
    • Hydroxyapatite fractionation of partially reassociated DNA was used to quantify repetitive DNA sequences.

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  • C14-labeled E. coli DNA was employed as a marker to control for non-specific binding during hydroxyapatite chromatography.

    • Main Results:

    • Approximately 20.2% of sheared chick DNA (1,300 nucleotide pairs) reassociated at a Cot value of 10, indicating the proportion of repetitive sequences.
    • The fraction of fast-reassociating (repetitive) sequences was consistent across all examined tissues and embryonic stages.
    • All reassociated DNA samples displayed identical Cesium Chloride (CsCl) density profiles, and repetitive sequences were found to be interspersed with non-repetitive DNA.

    Conclusions:

    • There is no evidence of specific DNA amplification of highly repetitive sequences in the somatic tissues of embryonic chicks studied.
    • The relative content of repetitive DNA is conserved across different embryonic tissues and developmental time points.
    • Repetitive DNA sequences in the chick genome are largely interspersed with unique sequences.