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Microfilament and microtubule function in human monocytes.

J J Rinehart, T Boulware

The Journal of Laboratory and Clinical Medicine

|October 1, 1977

Summary

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Microfilaments are crucial for human monocyte functions like mobility and phagocytosis. Microtubules play a role in monocyte chemotaxis, mobility, and adherence, but not in killing pathogens or engulfing particles.

Area of Science:

Immunology
Cell Biology

Background:

Human monocytes are key immune cells involved in pathogen clearance and inflammatory responses.
The roles of the cytoskeleton, specifically microfilaments and microtubules, in monocyte functions are not fully elucidated.

Purpose of the Study:

To investigate the distinct roles of microfilaments and microtubules in various human monocyte functions.

Main Methods:

Peripheral blood monocytes were treated with cytochalasin B (microfilament inhibitor) and colchicine (antitubulin).
Monocyte functions including chemotaxis, random mobility, candidacidal activity, phagocytosis, and adherence were measured.

Main Results:

Cytochalasin B significantly inhibited all tested monocyte functions (chemotaxis, mobility, candidacidal activity, phagocytosis, adherence).

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Colchicine significantly inhibited chemotaxis, random mobility, and adherence, but not phagocytosis or candidacidal activity.

Conclusions:

Microfilaments are essential for most human monocyte functions.
Microtubules are involved in monocyte membrane interactions during chemotaxis, mobility, and adherence.