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Related Experiment Videos

Characterization of Allomyces genome.

M Ojha, H Turler, G Turian

    Biochimica Et Biophysica Acta
    |October 18, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Allomyces arbuscula DNA analysis reveals distinct nuclear and extranuclear components. Genome size estimation shows a significant portion of highly and moderately repeated sequences in this fungal DNA.

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    Area of Science:

    • Molecular Biology
    • Genomics
    • Mycology

    Background:

    • Allomyces arbuscula DNA exhibits heterogeneity when isolated from whole cells.
    • Buoyant density centrifugation in neutral CsCl separates DNA into alpha, beta, and gamma components.

    Purpose of the Study:

    • To characterize the different DNA components of Allomyces arbuscula.
    • To determine the genome size and sequence organization of Allomyces arbuscula.

    Main Methods:

    • Buoyant density centrifugation in neutral CsCl.
    • DNA reassociation kinetics analysis of sheared, bulk, and nuclear DNA.
    • Sequence complexity determination relative to Escherichia coli.

    Main Results:

    • Bulk DNA contains alpha (nuclear), beta (mitochondrial), and gamma (extranuclear) components.

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  • Nuclear DNA primarily consists of the alpha component.
  • Reassociation kinetics indicate 25% highly repeated sequences in bulk DNA and 10% in nuclear DNA.
  • Moderately repeated sequences (15%) have a complexity of ~7.2x10^6 daltons, repeated ~320 times.
  • Approximately 60% of the genome consists of single-copy-like sequences.
  • The estimated genome size of Allomyces arbuscula is 1.7x10^10 daltons.

    • Conclusions:

    • The study elucidates the compositional heterogeneity of Allomyces arbuscula DNA.
    • Characterization of nuclear, mitochondrial, and other extranuclear DNA fractions provides insights into genome organization.
    • The genome size estimation and sequence complexity analysis contribute to understanding fungal genomics.