Related Experiment Videos
Mechanism by which fiber antigen inhibits multiplication of type 5 adenovirus
Abstract:
Purified fiber antigen of type 5 adenovirus inhibited the multiplication of type 5 adenovirus by 50% when 35 mug of fiber antigen protein was added to 10(6) KB cells in suspension culture. Although the fiber antigen reduced the number of virions adsorbed per cell when a multiplicity of infection of 50,000 plaque-forming units (PFU)/cell was employed, the number of cells infected was not diminished under these conditions. If a low multiplicity of infection (1.1 PFU/cell) was used, viral adsorption was not detectably decreased. The fiber antigen did not reduce the capability of virions to liberate their viral deoxyribonucleic acid (DNA). The biosyntheses of DNA, ribonucleic acid (RNA), and protein were blocked about 20 to 25 hr after the addition of fiber antigen to cultures of uninfected or type 5 adenovirus-infected KB cells. Most of the fiber antigen protein became cell-associated between 22 and 36 hr after it was added to cells. The hexon antigen neither inhibited viral multiplication nor blocked the biosynthesis of DNA, RNA, or protein. Moreover, the hexon did not attach to KB cells. The profound effects of the fiber antigen were not due to the induction of an interferon-like substance, for actinomycin D did not reduce the ability of the fiber to inhibit multiplication of type 1 poliovirus.
Insights
Type 5 adenovirus fiber antigen significantly inhibits viral multiplication and blocks host cell biosynthesis. Hexon antigen showed no such effects, indicating fiber antigen
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Adenovirus infections pose a significant public health challenge.
- Understanding viral replication mechanisms is crucial for developing antiviral therapies.
Purpose of the Study:
- To investigate the inhibitory effects of type 5 adenovirus fiber antigen on viral multiplication and host cell biosynthesis.
- To compare the effects of fiber antigen with hexon antigen on adenovirus replication.
Main Methods:
- Purified type 5 adenovirus fiber antigen was added to KB cell cultures.
- Viral multiplication, adsorption, and deoxyribonucleic acid (DNA) liberation were assessed.
- Biosynthesis of DNA, ribonucleic acid (RNA), and protein was monitored.
- Cell-association of antigens and induction of interferon-like substances were evaluated.
Main Results:
- Fiber antigen inhibited type 5 adenovirus multiplication by 50% at 35 µg/10^6 cells.
- Fiber antigen reduced virion adsorption at high multiplicity but not at low multiplicity.
- Viral DNA liberation was unaffected by fiber antigen.
- Fiber antigen blocked host cell DNA, RNA, and protein biosynthesis 20-25 hours post-addition.
- Hexon antigen showed no inhibitory effects and did not attach to cells.
- Fiber antigen's effects were not mediated by an interferon-like substance.
Conclusions:
- Type 5 adenovirus fiber antigen possesses potent antiviral activity by inhibiting viral multiplication and host cell macromolecular synthesis.
- Fiber antigen's mechanism of action involves interference with viral replication and host cell processes.
- Hexon antigen does not exhibit antiviral properties.
- Fiber antigen represents a potential target for antiviral drug development against adenovirus infections.