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Studies on human monocytes with a multiparameter cell sorter.

D Kwan, M B Epstein, A Norman

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1976
    PubMed
    Summary
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    Human monocytes, identified by their large volume and phagocytic activity, increase in number during incubation. These cells develop specific surface receptors, suggesting a developmental pathway from lymphocyte-like precursors.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Peripheral blood contains lymphocytes and monocytes with distinct volume distributions.
    • Monocytes are characterized by their phagocytic capacity and specific cell surface receptors.

    Purpose of the Study:

    • To characterize the volume distribution of human lymphocytes and monocytes.
    • To investigate the changes in monocyte number and characteristics during in vitro incubation.

    Main Methods:

    • Ficoll-hypaque density gradient centrifugation for cell separation.
    • Multiparameter cell sorter for Coulter volume distribution analysis.
    • Fluorescent latex particles for identifying phagocytic cells (monocytes).

    Main Results:

    • Human peripheral blood cell suspensions showed two main volume peaks: a major peak at 200 mu3 (lymphocytes) and a minor peak at 500 mu3 (monocytes).

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  • Monocytes were identified as the cells within the 500 mu3 peak, characterized by latex particle ingestion.
  • Incubation led to a doubling of monocyte numbers within 3 days, accompanied by an increase in average cell volume.
  • Mature monocytes after incubation lacked sheep red blood cell receptors but possessed human gamma-globulin receptors.

    • Conclusions:

    • Monocyte proliferation in vitro appears to originate from lymphocyte-like precursors that enlarge and acquire monocyte characteristics.
    • These precursors possess receptors for both sheep red blood cells and human gamma-globulin, similar to monocytes and B lymphocytes.
    • The study elucidates a potential developmental pathway for monocytes from precursor cells within peripheral blood suspensions.