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A new alloantigenic system associated with the Mls locus in the mouse
Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1976
Summary
Researchers identified a new mouse alloantigen system, AST-101, which targets B cells and phagocytes. This system is genetically linked to the mouse minor MLC-stimulating (Mls) locus, suggesting a novel cell surface marker.
Area of Science:
- Immunology
- Genetics
- Cell Biology
Background:
- A novel antiserum, AST-101, was generated using mouse tissue.
- This antiserum reacts with previously undescribed cell surface alloantigens.
Purpose of the Study:
- To characterize the specificity and genetic linkage of the AST-101 alloantigen system.
- To determine the cell types targeted by AST-101.
Main Methods:
- Cytotoxic assays using lymphoid cells and peritoneal exudate cells.
- Strain distribution analysis to assess linkage with known mouse alloantigen systems (H-2, Ly, Thy).
- Genetic analysis to determine linkage with the minor MLC-stimulating (Mls) locus.
Main Results:
- AST-101 selectively kills B cells but not T cells in cytotoxic tests.
- Phagocytic cells from peritoneal exudates are also sensitive to AST-101 and complement.
- Genetic analysis revealed close linkage between the AST-101 system and the Mls locus.
- Serologic analysis confirmed an association between AST-101 reactive antigens and Mls gene products.
Conclusions:
- AST-101 defines a new mouse alloantigen system.
- The AST-101 system is closely linked to the Mls locus, suggesting a shared genetic basis or functional relationship.
- This discovery provides new tools for studying B cell and phagocyte populations and their relationship to Mls antigens.