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Related Experiment Videos

Free testosterone concentration in serum: a method for determination.

W G Wiest, J D Paulson, D W Keller

    American Journal of Obstetrics and Gynecology
    |February 1, 1978
    PubMed
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    Two methods were evaluated to measure testosterone-estradiol-binding globulin (TeBG) for estimating serum free testosterone. The DEAE-cellulose filtration method is preferred for clinical assessment of androgen levels.

    Area of Science:

    • Endocrinology
    • Biochemistry

    Background:

    • Accurate measurement of serum free testosterone is crucial for assessing androgen status.
    • Existing methods for quantifying testosterone-estradiol-binding globulin (TeBG) require evaluation for clinical feasibility.

    Purpose of the Study:

    • To evaluate two indirect procedures for quantifying serum TeBG levels.
    • To develop a clinically feasible method for estimating serum free testosterone.

    Main Methods:

    • Method 1: Varied serum protein concentration and used dextran-coated charcoal separation.
    • Method 2: Utilized DEAE-cellulose filtration to isolate and quantify the TeBG-steroid complex.

    Main Results:

    • Both methods assessed protein-steroid interaction indirectly.

    Related Experiment Videos

  • DEAE-cellulose filtration proved superior for routine clinical evaluation.
  • Accurate TeBG measurement, combined with total testosterone, allows free testosterone estimation.

    • Conclusions:

    • The DEAE-cellulose filtration method is a reliable and clinically useful tool.
    • This method facilitates the routine evaluation of physiologically effective androgen levels.