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RELATIONSHIP BETWEEN THE Mason-Pfizer monkey virus and Hela virus: immunoelectron microscopy
Abstract:
Envelope antigens of the Mason-Pfizer monkey virus (MPMV) and the morphologically similar HeLa virus, which is continuously produced in some HeLa cell lines, were compared by indirect immunoferritin techniques. Antisera raised against MPMV and HeLa virus revealed cross-reacting antigenic specificities on the surfaces of both agents, and cross-absorption and end-point dilution techniques indicated the presence of identical envelope antigens. The results demonstrated a close relationship, if not an identity, between MPMV and HeLa virus.
Insights
Mason-Pfizer monkey virus (MPMV) and HeLa virus share identical envelope antigens. This suggests a very close relationship, potentially even identity, between these two viruses based on their surface proteins.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Mason-Pfizer monkey virus (MPMV) is a known simian retrovirus.
- HeLa virus is a contaminant observed in some HeLa cell lines.
- Morphological similarities exist between MPMV and HeLa virus.
Purpose of the Study:
- To compare the envelope antigens of MPMV and HeLa virus.
- To determine the antigenic relationship between MPMV and HeLa virus.
Main Methods:
- Indirect immunoferritin techniques were employed.
- Antisera against MPMV and HeLa virus were used.
- Cross-absorption and end-point dilution assays were performed.
Main Results:
- Cross-reacting antigenic specificities were detected on the surfaces of both MPMV and HeLa virus.
- Cross-absorption and end-point dilution indicated identical envelope antigens.
- MPMV and HeLa virus share common surface proteins.
Conclusions:
- MPMV and HeLa virus exhibit a close antigenic relationship.
- The envelope antigens suggest that MPMV and HeLa virus may be identical.