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Updated: Jul 31, 2026
11:01
Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
Published on: September 25, 2016
Improvement of a continuous-culture apparatus for long-term use
Applied Microbiology
|February 1, 1968
Abstract:
A glass and plastic apparatus was designed to meet requirements for continuous culture of cells. Some of the improvements incorporated into this apparatus include an all-glass growth vessel with a self-cleaning bottom, special compression fittings to connect tubing to glass tubing, a removable yield reservoir, and a nonwetting gas exhaust assembly. All portions of the system can be autoclaved for sterilization, and medium bottles and pump lines are replaced aseptically.
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