Application of the egg-bit technique in poultry-disease research

Experiments were carried out to determine the usefulness of the egg-bit technique (EBT) in poultry virus research with various agents available on the market. The EBT was found satisfactory for propagating 9 strains of avian influenza virus of different antigenic groups, with only minor differences resulting when the procedure used two sources of gelatin, presence or absence of CaCl2, or commercial or specific-pathogen-free embryos.