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Genetic differences in mouse hemoglobin were observed between DBA/C57B1 and C3H/C57B1 strains. Fetal and adult globin chains show distinct electrophoretic mobilities, suggesting close linkage of fetal and adult beta-chain loci.
Area of Science:
- Genetics
- Molecular Biology
- Hematology
Background:
- Hemoglobin composition varies across mouse strains.
- Fetal and adult globin chains exhibit strain-specific electrophoretic properties.
Purpose of the Study:
- To investigate genetic differences in fetal and adult hemoglobin chains among mouse strains.
- To determine the genetic linkage between fetal globin and adult beta-globin loci.
Main Methods:
- Electrophoretic analysis of fetal and adult globin chains in DBA, C3H, and C57B1 mice.
- Backcrossing hybrid mice ((DBA x C57B1) x C57B1) to assess genetic linkage.
Main Results:
- DBA and C3H mice possess fetal globin chains with different electrophoretic mobilities compared to C57B1 mice.
- DBA and C3H mice exhibit distinct adult hemoglobin types compared to C57B1 mice.
- Backcrossing data indicate close genetic linkage between the fetal globin chain locus and the adult beta-chain locus.
Conclusions:
- Mouse strains DBA and C3H show unique fetal and adult hemoglobin chain characteristics.
- The genetic loci for fetal globin and adult beta-globin chains are closely linked in mice.
Abstract:
Two strains of mice, DBA and C3H, have a fetal globin polypeptide chain which differs in electrophoretic mobility from the corresponding fetal chain of the C57B1 strain. Mice of the DBA and C3H strains also differ from those of the C57B1 in adult hemoglobin type. Results of backcrossing the (DBA x C57B1) hybrid to the C57B1 suggest that the fetal chain locus and the adult beta-chain locus are closely linked.