Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Immunologic specificity of local inhibitory intercellular interactions].

O S Grigor'eva, A A Korukova, A E Gurvich

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |June 1, 1978
    PubMed
    Summary

    Local cell interactions in mouse spleens suppress antibody-forming cell (AFC) growth. Prior immunization specifically blocks this suppression for the targeted antigen, revealing immune memory's role.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Production and characterization of monoclonal antibodies to group-specific antigenic determinant of group A streptococcal polysaccharides].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2001
    Same author

    [Production and characteristics of monoclonal antibodies against group-specific antigen determinant of Streptococcus group A polysaccharide].

    Biulleten' eksperimental'noi biologii i meditsiny·1999
    Same author

    [Specificity of a panel of monoclonal antibodies to group A Streptococcus polysaccharide and search for cross reactions with mammalian tissues].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1999
    Same author

    [Secondary immune response of mice immunized with a protein-cellulose complex].

    Biulleten' eksperimental'noi biologii i meditsiny·1986
    Same author

    [Cultivation of cells on modified porous cellulose beads].

    Biulleten' eksperimental'noi biologii i meditsiny·1986
    Same author

    Induction of abundant antibody formation with a protein-cellulose complex in mice.

    Journal of immunological methods·1986

    Area of Science:

    • Immunology
    • Cell Biology
    • Spleen Cell Culture

    Background:

    • Previously identified local intercellular interactions in mouse spleen cell suspensions inhibit antibody-forming cell (AFC) proliferation.
    • This inhibition is density-dependent in non-immunized mice.
    • The mechanism of this density-dependent inhibition was not fully understood.

    Purpose of the Study:

    • To investigate the effect of prior antigen immunization on AFC proliferation inhibition.
    • To determine if immunization influences antigen-specific or non-specific cell interactions.
    • To assess the impact on general cell proliferation inhibition.

    Main Methods:

    • Cultivation of mouse spleen cell suspensions at varying densities.
    • Preliminary immunization of mice with a specific antigen.

    Related Experiment Videos

  • Assessment of AFC count and cell proliferation inhibition in cultures from immunized and non-immunized mice.

    • Main Results:

    • Preliminary immunization eliminated or significantly reduced AFC proliferation inhibition for the specific antigen.
    • Immunization did not affect inhibition of AFCs producing antibodies against a different antigen.
    • General proliferation inhibition of bulk cells remained unaffected by immunization.

    Conclusions:

    • Prior immunization induces antigen-specific tolerance to local intercellular inhibitory mechanisms.
    • The observed inhibition is specific to AFCs responding to the immunizing antigen.
    • This suggests a role for immune memory in regulating cellular interactions within the spleen.