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Terbium as a solid-state probe for RNA.

G Yonuschot, D Helman, G Mushrush

    Bioinorganic Chemistry
    |January 1, 1978
    PubMed
    Summary
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    This study reveals that terbium ions bind to nucleic acids via phosphate groups and guanine moieties enhance fluorescence. This allows for picomole-level quantification of DNA and RNA using solid-state complexes.

    Area of Science:

    • Biochemistry
    • Spectroscopy
    • Materials Science

    Background:

    • Previous research explored nucleic acid-terbium complexes in solid states.
    • Understanding the interaction between nucleic acids and terbium ions is crucial for developing new analytical methods.

    Purpose of the Study:

    • To analyze the fluorescence spectra of RNA-terbium(III) and DNA-terbium(III) complexes.
    • To investigate the binding mechanisms and energy transfer processes in these complexes.
    • To establish a method for quantifying DNA and RNA using these complexes.

    Main Methods:

    • Characterization of fluorescence excitation and emission spectra.
    • Trapping of nucleic acid-terbium complexes on millipore filters.
    • SDS-extraction for recovery of deoxyribonucleic acid.

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  • Binding studies using radioactive terbium.

    • Main Results:

    • Complete trapping of DNA-terbium complexes on millipore filters was achieved.
    • Energy transfer from nucleic acid bases to terbium ions was confirmed.
    • Phosphate groups were identified as the primary binding sites for terbium.
    • The guanine moiety was found to be the main contributor to observed fluorescence.
    • Equal affinity of terbium for all homopolyribonucleotides was observed.

    Conclusions:

    • Terbium ions bind to nucleic acids through phosphate groups, with guanine enhancing fluorescence.
    • Solid-state DNA and RNA terbium complexes can be used to accurately measure picomole quantities of nucleic acids.