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Uridylate kinase activity: effect of isoproterenol
Science (New York, N.Y.)
|October 18, 1968
Abstract:
Isoproterenol stimulates cell proliferation in mouse salivary glands. Prior to the stimulation of DNA synthesis, (3)H-uridine incorporation into RNA is decreased. This decreased incorporation results from a depression of uridylate kinase activity.
Insights
Isoproterenol triggers cell growth in mouse salivary glands. This occurs because uridylate kinase activity decreases, reducing RNA synthesis before DNA synthesis begins.
Area of Science:
- Biochemistry
- Cell Biology
- Physiology
Background:
- Isoproterenol is a known stimulant of salivary gland growth.
- Cell proliferation involves complex regulatory mechanisms controlling DNA and RNA synthesis.
Purpose of the Study:
- To investigate the early molecular events preceding DNA synthesis stimulation by isoproterenol in mouse salivary glands.
- To identify the specific enzymatic activities affected during the initial phase of isoproterenol-induced cell proliferation.
Main Methods:
- Administration of isoproterenol to mouse salivary glands.
- Measurement of (3)H-uridine incorporation into RNA.
- Assay of uridylate kinase activity.
Main Results:
- Isoproterenol stimulated cell proliferation.
- A decrease in (3)H-uridine incorporation into RNA was observed prior to DNA synthesis stimulation.
- Uridylate kinase activity was found to be depressed during this period.
Conclusions:
- The initial response to isoproterenol involves a depression of uridylate kinase activity.
- This enzymatic depression leads to reduced RNA synthesis, preceding the onset of DNA synthesis and cell proliferation.