Related Experiment Video
Updated: Aug 8, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Published on: November 7, 2012
[Action of egg lysozyme on representatives of the family Micrococcaceae. Its action on Micrococcus]
Abstract:
The results of the study of the effect of various concentrations of egg lysozyme on M. luteus and M. varians using 2 methods, i.e. serial dilutions in agar and turbidimetric are presented. It was found that the MIC of lysozyme for M. luteus ranged within wide limits, from less than 0.0003 to 1 mg/ml. M. varians was stable to lysozyme. The MIC for all the strains was 8 mg/ml. The turbidimetric method provided determination of general regularities in changes of the optical density in all the strains of M. luteus under the effect of various concentrations of lysozyme. On the basis of these data it was possible to consider the method as the most deep means for determining the intraspecies similarities in the surface structures of Micrococcus as compared to the method of serial dilutions in agar. The dynamics of the changes in the optical density of the M. luteus suspension markedly differed from that of M. varians.
Related Concept Videos
Lysosomal Hydrolases
Chemical Agents for Microbial Control
Biological Methods for Microbial Control
Antimicrobial Effectiveness
Viral Replication: Lytic Cycle
Inhibitors of Gram-positive Cell Wall Synthesis

