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A specific radio-immunological assay for prednisolone in plasma
Research Communications in Chemical Pathology and Pharmacology
|September 1, 1978
Abstract:
A new, specific radio-immunological assay (R.I.A.) for prednisolone in plasma is described. The R.I.A. incorporates a procedure whereby the endogenous interfering hydrocortisone (in de-proteinized plasma) reacts directly with Girard Reagent T, resulting in a derivative which does not cross react with the specific antibody for prednisolone. The assay is sensitive (quantitation levels are in the 5-10 ng/ml range). The specificity compares favorably with that of a high performance liquid chromatographic procedure.