Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunological phenomena induced by UV rays.

M Jarzabek-Chorzelska, Z Zarebska, H Wolska

    Acta Dermato-Venereologica
    |January 1, 1976
    PubMed
    Summary

    Researchers detected UV-induced DNA damage in mouse skin using immunofluorescence. The damage repair occurred within 48 hours, suggesting efficient DNA repair mechanisms in epidermal cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Is palmoplantar pustulosis simply a variant of psoriasis or a distinct entity?

    Journal of the European Academy of Dermatology and Venereology : JEADV·2017
    Same author

    What do we know about palmoplantar pustulosis?

    Journal of the European Academy of Dermatology and Venereology : JEADV·2016
    Same author

    Pemphigus herpetiformis with IgA and IgG antibodies to desmoglein 1 and IgG antibodies to desmocollin 3.

    Journal of the American Academy of Dermatology·2003
    Same author

    Immunopathological diagnosis of pemphigus foliaceus.

    Dermatology (Basel, Switzerland)·2002
    Same author

    Celiac disease and immunoglobulin a deficiency: how effective are the serological methods of diagnosis?

    Clinical and diagnostic laboratory immunology·2002
    Same author

    The use of two substrates for indirect immunofluorescence in the diagnosis of pemphigus.

    The British journal of dermatology·2001

    Area of Science:

    • Photochemistry
    • Molecular Biology
    • Dermatology

    Background:

    • Ultraviolet (UV) radiation causes DNA damage in skin cells.
    • Detecting and understanding the repair of this damage is crucial for skin health.
    • Previous methods lacked specificity for identifying UV-induced DNA lesions.

    Purpose of the Study:

    • To develop and utilize a specific method for detecting UV-induced DNA damage in vivo.
    • To investigate the time course of DNA damage and repair in mouse epidermis after UV exposure.
    • To explore the relationship between DNA repair and epidermal cell cycling.

    Main Methods:

    • Preparation of antiserum against UV-irradiated DNA.
    • Application of indirect immunofluorescence (IF) technique.
    • Microscopic examination of hairless mouse dorsal epidermis sections post-irradiation.

    Main Results:

    • UV-induced DNA damage was detected via nuclear fluorescence immediately after irradiation.
    • This fluorescence, indicating DNA damage, persisted for 24 hours.
    • The immunofluorescence reaction became negative within 48 hours, regardless of UV dose.
    • No DNA changes were observed with UV doses similar to sunlight's erythema spectrum (up to 40 MED).

    Conclusions:

    • The rapid disappearance of detectable DNA damage suggests efficient DNA repair processes in mouse epidermis.
    • The timing of repair may be linked to the epidermal cell cycle.
    • The developed immunofluorescence method is sensitive for detecting UV-induced DNA photoproducts.

    Related Experiment Videos