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Sterilization of hepatitis B surface antigen-contaminated materials
Medical Instrumentation
|May 1, 1978
Summary
Boiling and steam effectively inactivate Hepatitis B surface antigen (HBs Ag) in serum. However, HBs Ag-contaminated materials require 30 minutes at 132°C using steam under pressure for sterilization.
Area of Science:
- Infectious disease control
- Biomedical engineering
- Sterilization technologies
Background:
- Hepatitis B virus (HBV) poses a significant risk in healthcare settings.
- Effective sterilization of contaminated materials is crucial for preventing HBV transmission.
- Hepatitis B surface antigen (HBs Ag) is a key indicator of HBV infectivity.
Purpose of the Study:
- To determine optimal sterilizing conditions for HBs Ag-contaminated materials.
- To evaluate the inactivation of HBs Ag antigenicity under various sterilization methods.
- To establish effective protocols for healthcare-associated infection control.
Main Methods:
- Utilized HBs Ag-coated beads and HBs Ag-positive patient serum as test materials.
- Applied various sterilization procedures including boiling and steam under pressure.
- Assessed HBs Ag antigenicity post-sterilization using radioimmunoassay.
Main Results:
- HBs Ag-positive serum was readily inactivated by boiling and standard steam sterilization.
- HBs Ag-coated beads demonstrated greater resistance, requiring enhanced steam under pressure.
- A validated sterilization protocol involves 30 minutes at 132°C with steam under pressure.
Conclusions:
- Sterilization protocols must account for the physical state of HBs Ag contamination.
- Enhanced steam under pressure is necessary for materials with adsorbed HBs Ag.
- Recommended sterilization: 30 minutes at 132°C for HBs Ag-contaminated medical materials.