Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental scrapie in mice: ultrastructural observations.

J R Baringer, S B Prusiner

    Annals of Neurology
    |September 1, 1978
    PubMed
    Summary

    Researchers observed unique 23 nm particles in the brains of mice infected with the scrapie agent. These particles appeared consistently in infected mice, suggesting a potential role in spongiform encephalopathies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Copper induces conformational changes in the N-terminal part of cell-surface PrPC.

    Archives of virology·2006
    Same author

    MRI abnormalities in chronic active varicella zoster infection.

    Neurology·2004
    Same author

    Engineering the prion protein using chemical synthesis.

    The journal of peptide research : official journal of the American Peptide Society·2002
    Same author

    Inherited prion disease caused by the V210I mutation: transmission to transgenic mice.

    Neurology·2002
    Same author

    Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein.

    Journal of molecular biology·2001
    Same author

    Doppel-induced cerebellar degeneration in transgenic mice.

    Proceedings of the National Academy of Sciences of the United States of America·2001

    Area of Science:

    • Neuroscience
    • Pathology
    • Microbiology

    Background:

    • Scrapie, kuru, and Creutzfeldt-Jakob disease share spongiform pathology and prolonged incubation periods.
    • These transmissible spongiform encephalopathies are associated with an unconventional infectious agent.

    Purpose of the Study:

    • To investigate the ultrastructural changes in the brains of scrapie-infected mice.
    • To identify potential physical characteristics of the scrapie agent at the microscopic level.

    Main Methods:

    • Swiss mice were inoculated with the scrapie agent.
    • Brain tissue was examined using light and electron microscopy at three and five months post-inoculation.

    Main Results:

    • Vacuoles in the neuropil and astrocytic proliferation were observed, increasing in severity by five months.
    • Unique 23 nm osmiophilic particles were consistently found within postsynaptic processes of infected mouse brains, but not in controls.

    Conclusions:

    • The observed 23 nm particles in postsynaptic processes are a consistent feature of scrapie infection in mice.
    • These particles' size and consistent presence align with expectations for the scrapie agent, warranting further investigation.

    Related Experiment Videos