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Disk agar diffusion susceptibility testing of yeasts.

M A Saubolle, P D Hoeprich

    Antimicrobial Agents and Chemotherapy
    |October 1, 1978
    PubMed
    Summary
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    A new disk agar diffusion method offers a reliable way to test yeast susceptibility to antifungal drugs. This method provides accurate results for common yeasts like Candida, aiding clinical decisions.

    Area of Science:

    • Clinical Microbiology
    • Mycology
    • Antimicrobial Susceptibility Testing

    Background:

    • Rapidly growing yeasts, including Candida spp. and Torulopsis glabrata, are significant human pathogens.
    • Accurate in vitro susceptibility testing is crucial for guiding antifungal therapy.
    • Established methods like broth and agar dilution can be time-consuming or resource-intensive.

    Purpose of the Study:

    • To develop and validate a disk agar diffusion method for assessing the susceptibility of rapidly growing yeasts.
    • To determine optimal conditions and drug concentrations for disk diffusion testing.
    • To correlate disk diffusion results with established quantitative methods (broth and agar dilution).

    Main Methods:

    • A defined synthetic amino acid medium was used for yeast culture.

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  • Standardized inocula were prepared using turbidimetric adjustment of cell suspensions.
  • Disk diffusion assays were performed with optimized drug loads for five antifungal agents, and zones of inhibition were measured.

    • Main Results:

    • The disk diffusion method produced sharp, reproducible zones of inhibition.
    • Disk potencies remained stable for two months when stored appropriately.
    • High correlation rates were observed between disk diffusion zone diameters and minimum inhibitory concentrations (MICs) determined by broth and agar dilution for most antifungals tested.

    Conclusions:

    • The developed disk agar diffusion method is a generally applicable and reliable tool for yeast susceptibility testing.
    • The method demonstrates good agreement with quantitative MIC determination, offering a potentially faster alternative.
    • Quantitative methods should still be employed when disk diffusion results are equivocal.