A scanning electron microscopic study of the neural crest cell cytoskeleton after triton x-100 demebranation

Triton x-100, which solubilizes cell membranes, was used to expose the detergent resistant cytoskeletal systems of cultured day 4 (angulated) and a day 8 (STELLATE) NEURAL CREST cells. This procedure reveals a network of fibers which correlates structurally with the morphological state of neural crest cell differentiation.