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Related Experiment Videos

Phenotypic mixing between two primate oncoviruses.

T J Schnitzer

    The Journal of General Virology
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Phenotypic mixing between primate oncoviruses HL23V and BEV created a pseudotype virus. This allowed HL23V to infect previously resistant mouse cells, expanding its host range without genetic recombination.

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    Alimentary pharmacology & therapeutics·2015

    Area of Science:

    • Virology
    • Oncoviruses
    • Cell Biology

    Background:

    • Primate oncoviruses like HL23V and BEV can interact within host cells.
    • Understanding viral host range and tropism is crucial for virology research.
    • Previous studies indicated resistance of murine cells to HL23V replication.

    Purpose of the Study:

    • To investigate phenotypic mixing between HL23V and BEV.
    • To determine if this interaction affects viral host range and genetic material exchange.
    • To analyze the replication capabilities of pseudotype viruses in different cell types.

    Main Methods:

    • Co-cultivation of bat lung (Tb) cells doubly infected with HL23V and BEV.
    • Production and characterization of HL23V(BEV) pseudotype virus.

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  • Replication assays in murine and rat cell lines, including MSV-transformed non-producer cells.
  • Analysis of viral genetic information expression.

    • Main Results:

    • Phenotypic mixing occurred, producing HL23V(BEV) pseudotype virus.
    • The HL23V(BEV) pseudotype successfully replicated in murine cells, overcoming a prior penetration block.
    • BEV genetic information was expressed in doubly-infected rat cells and co-cultivated mouse/rat cell lines.
    • No evidence of genetic recombination between HL23V and BEV was detected.

    Conclusions:

    • Phenotypic mixing can alter the host range of oncoviruses.
    • Pseudotype formation by HL23V and BEV enables infection of resistant murine cells.
    • HL23V and BEV interact via phenotypic mixing, not genetic recombination, in this model.