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A simple circuit for automatic continuous recording of microelectrode resitance.

G R Naylor

    Pflugers Archiv : European Journal of Physiology
    |December 28, 1978
    PubMed
    Summary
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    Researchers developed a circuit to monitor microelectrode resistance during intracellular recordings. This helps prevent artefactual readings caused by tip potentials, improving data accuracy in electrophysiology.

    Area of Science:

    • Neuroscience
    • Biophysics
    • Electrophysiology

    Background:

    • Intracellular recording using microelectrodes (<0.5 micron tip diameter) can yield artefactual readings.
    • These artifacts are often attributed to tip potentials, which correlate with changes in microelectrode resistance upon cell impalement.

    Purpose of the Study:

    • To describe a circuit for continuous monitoring of microelectrode resistance during experiments.
    • To mitigate artefactual readings in intracellular recordings by tracking electrode resistance.

    Main Methods:

    • A circuit was designed utilizing the microelectrode as part of an AC potential divider (20 Hz, 20 mV amplitude).
    • A 1000 MΩ resistor formed the other half of the potential divider.
    • The AC signal across the microelectrode, proportional to its resistance (0-50 MΩ), was amplified, rectified, smoothed, and displayed.

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    Main Results:

    • The described circuit allows for real-time monitoring of microelectrode resistance.
    • Continuous resistance monitoring can identify and potentially correct for tip-potential-induced artifacts.

    Conclusions:

    • The developed circuit provides a reliable method for assessing microelectrode integrity during electrophysiological recordings.
    • This technique enhances the accuracy and reliability of intracellular recording data by accounting for electrode resistance variations.