Vaccinia virus replication and cytopathic effect in cultures in phytohemagglutinin-treated human peripheral blood...

Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell injury, decline in cell numbers, and decreased acid production. Leukocytes not pretreated with phytohemagglutinin appeared to form infective centers after exposure to the vaccinia agent, but they did not replicate infectious virus. For viral replication, the continuous presence of phytohemagglutinin was required.