Related Concept Videos

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier09:00

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.
20.8K
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines09:49

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to...
15.4K
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes07:29

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or...
20.4K
Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis04:37

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Exercise is capable of inducing apoptosis in immune cells. There are various measurement limitations, particularly relating to the amount of time required to isolate and treat a blood sample prior to the assessment. Demonstrated is a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte...
12.5K
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies11:52

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of...
18.6K
Delivering Effector T Lymphocytes into Mice Carrying Human Brain Tumors04:50

Delivering Effector T Lymphocytes into Mice Carrying Human Brain Tumors

This video demonstrates a stereotaxic injection technique for administering human effector T lymphocytes into a murine model carrying a human brain tumor. Upon positioning a tumor-bearing anesthetized mouse on a stereotactic frame and creating a burr hole at the injection location on the skull, effector T lymphocytes are injected at the tumor...
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