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Related Experiment Videos

Effect of morphine on synaptosomal Ca++ uptake.

F Guerrero-Munoz, K V Cerreta, M L Guerrero

    The Journal of Pharmacology and Experimental Therapeutics
    |April 1, 1979
    PubMed
    Summary

    Morphine affects calcium uptake in mouse brain synaptosomes. Acute morphine decreases uptake, while chronic use enhances it, suggesting calcium

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Biochemistry

    Background:

    • Calcium ions (Ca++) play a crucial role in neurotransmitter release and neuronal signaling.
    • Morphine, an opioid analgesic, exerts its effects through complex interactions within the central nervous system.
    • Synaptosomes, isolated nerve terminals, are valuable models for studying neuronal function and drug effects.

    Purpose of the Study:

    • To investigate the impact of morphine on calcium-45 (45Ca++) uptake in mouse brain synaptosomes.
    • To explore the role of calcium fluxes in the development of morphine tolerance and physical dependence.

    Main Methods:

    • Utilized two distinct methods for measuring 45Ca++ uptake: centrifugation and filtration.
    • Administered morphine acutely (subcutaneously) and chronically (pellet implantation) to mice.
    • Investigated the effects of naloxone, an opioid antagonist, on morphine-induced changes in 45Ca++ uptake.

    Main Results:

    • Morphine addition in vitro and acute in vivo treatment significantly reduced 45Ca++ uptake in synaptosomes.
    • Naloxone administration reversed the inhibitory effect of morphine on 45Ca++ uptake.
    • Chronic morphine treatment led to an enhancement of 45Ca++ uptake in synaptosomes.

    Conclusions:

    • Changes in synaptosomal calcium fluxes are implicated in the pharmacological actions of morphine, including analgesia.
    • Altered calcium handling in neurons may contribute to the development of morphine tolerance and physical dependence.
    • These findings highlight the critical role of calcium homeostasis in opioid drug effects.

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