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Bovine coronavirus genome.

J S Guy, D A Brian

    Journal of Virology
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Bovine coronavirus RNA, isolated from the Mebus strain, is a large, homogeneous molecule. This RNA exhibits partial resistance to RNase A and significant polyadenylation, indicating complex genomic properties.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Biochemistry

    Background:

    • Bovine coronavirus (BCV) is an important veterinary pathogen.
    • Understanding the molecular characteristics of viral RNA is crucial for developing antiviral strategies.

    Purpose of the Study:

    • To characterize the RNA genome of a tissue culture-adapted strain of bovine coronavirus (Mebus).
    • To determine the molecular weight, stability, nuclease susceptibility, and polyadenylation of BCV RNA.

    Main Methods:

    • Isolation and purification of RNA from Mebus strain BCV.
    • Isotopic labeling of RNA with [3H]uridine.
    • Electrophoresis to determine molecular weight.
    • Heat denaturation to assess RNA stability.
    • RNase A digestion to evaluate nuclease susceptibility.

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  • Binding assays with polyuridylic acid-Sepharose and oligodeoxythymidylic acid-cellulose to assess polyadenylation.

    • Main Results:

    • BCV RNA is a large, homogeneous molecule with an apparent molecular weight of 3.8 x 10^6.
    • The RNA remained stable after heat denaturation.
    • Approximately 80% of the RNA was susceptible to RNase A digestion, with a resistant fraction of 4S-7S.
    • The RNA was polyadenylated, with 40% binding to polyuridylic acid-Sepharose and 60% binding to oligodeoxythymidylic acid-cellulose.

    Conclusions:

    • The Mebus strain of BCV possesses a large, predominantly single-stranded RNA genome.
    • The RNA genome shows partial resistance to RNase A and significant polyadenylation.
    • These findings contribute to the understanding of bovine coronavirus molecular biology.