Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in diffraction patterns with length in single muscle fibres at rest.

Y M Cheung, J C Hwang, S H Chung

    Pflugers Archiv : European Journal of Physiology
    |February 14, 1979
    PubMed
    Summary

    Researchers developed a new method to measure laser diffraction patterns in frog muscle fibers. This technique confirmed maximum intensity at 2.95-3.00 microns and revealed a linear relationship between sarcomere dispersion and length.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Multi-peak Light-emitting Diode Curing Units and Polymerization of 3D-printed Crown and Bridge Resin.

    Operative dentistry·2023
    Same author

    Steroid-Refractory Autoimmune Myocarditis after Pembrolizumab Therapy: Failure of Equine Anti-Thymocyte Globulin to Prevent Heart Failure.

    Journal of clinical case reports·2023
    Same author

    Observation of a 1/3 magnetization plateau in Pb<sub>2</sub>Cu<sub>10</sub>O<sub>4</sub>(SeO<sub>3</sub>)<sub>4</sub>Cl<sub>7</sub> arising from (Cu<sup>2+</sup>)<sub>7</sub> clusters of corner-sharing (Cu<sup>2+</sup>)<sub>4</sub> tetrahedra.

    Dalton transactions (Cambridge, England : 2003)·2022
    Same author

    Utility of the Addenbrooke's Cognitive Examination III online calculator to differentiate the primary progressive aphasia variants.

    Brain communications·2022
    Same author

    Effect of Deep Margin Elevation on Interfacial Gap Development of CAD/CAM Inlays after Thermomechanical Cycling.

    Operative dentistry·2021
    Same author

    A novel model to manage air cargo disruptions caused by global catastrophes such as Covid-19.

    Journal of air transport management·2021

    Area of Science:

    • Muscle physiology
    • Biophysics
    • Optical measurement techniques

    Background:

    • Understanding muscle fiber mechanics is crucial for physiology.
    • Laser diffraction is a key technique for analyzing muscle structure.
    • Previous methods had limitations in measuring fine details of muscle fibers.

    Purpose of the Study:

    • To develop and validate a novel method for measuring laser diffraction patterns in single frog muscle fibers.
    • To precisely determine the sarcomere length and dispersion in resting muscle fibers.
    • To investigate the relationship between sarcomere dispersion and sarcomere length.

    Main Methods:

    • Integration of an X-Y sampling circuit with a closed-circuit television (CCTV) system.
    • Acquisition of laser diffraction patterns from isolated, resting single frog muscle fibers.

    Related Experiment Videos

  • Analysis of diffraction patterns to determine sarcomere length and dispersion.

    • Main Results:

    • The study successfully measured narrow profiles of laser diffraction patterns.
    • Maximum diffraction intensity was confirmed at a sarcomere length of 2.95-3.00 microns.
    • A positive linear relationship was observed between sarcomere dispersion and sarcomere length (2.7-3.8 microns) in four fibers.

    Conclusions:

    • The developed X-Y sampling circuit enhances laser diffraction analysis of muscle fibers.
    • The findings provide precise quantitative data on sarcomere length and dispersion in resting frog muscle.
    • This method offers a valuable tool for future studies in muscle biophysics.