[Separation of human X- and Y- chromosome-bearing spermatozoa by column chromatography (author's transl)]

Separation of X- and Y-chromosome-bearing spermatozoa has been attempted using Sephadex gel filtration. Fractions rich in X-chromosome-bearing spermatozoa were eluted in dependence on length of separation distance and on speed of elution. Spermatozoa remaining at the gel showed a higher percentage of Y-bodies compared with the untreated control. Sedimentation pattern and amount of eluted cells is influenced by eluation conditions.