Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzymic assay for serum theophylline.

B Vinet, L Zizian

    Clinical Chemistry
    |August 1, 1979
    PubMed
    Summary

    A new method accurately measures theophylline in serum using alkaline phosphatase inhibition. This cost-effective procedure offers stable reagents and simple instrumentation for reliable drug monitoring.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Training of residents in obstetrical-gynaecology in Toulouse].

    Gynecologie, obstetrique & fertilite·2011
    Same author

    Atomised lidocaine for airway topical anaesthesia in the morbidly obese: 1% compared with 2%.

    Anaesthesia·2009
    Same author

    Airway topicalisation in morbidly obese patients using atomised lidocaine: 2% compared with 4%.

    Anaesthesia·2007
    Same author

    In vivo higher glucuronidation of mycophenolic acid in male than in female recipients of a cadaveric kidney allograft and under immunosuppressive therapy with mycophenolate mofetil.

    Therapeutic drug monitoring·2001
    Same author

    Stability of PO2, PCO2, and pH in fresh blood samples stored in a plastic syringe with low heparin in relation to various blood-gas and hematological parameters.

    Clinical biochemistry·1999
    Same author

    Valproic acid intoxication in a patient with bipolar disorder and chronic uremia.

    Canadian journal of psychiatry. Revue canadienne de psychiatrie·1999

    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Pharmacology

    Background:

    • Accurate theophylline monitoring is crucial for patient safety and therapeutic efficacy.
    • Existing methods may have limitations in cost, complexity, or interference.

    Purpose of the Study:

    • To develop and validate a novel, cost-effective assay for serum theophylline determination.
    • To assess the accuracy, reliability, and interference profile of the new method.

    Main Methods:

    • Serum theophylline extraction using chloroform/isopropanol and back-extraction into alkaline solution.
    • Measurement of theophylline-induced inhibition of beef-liver alkaline phosphatase activity.
    • Enzyme activity assay using p-nitrophenyl phosphate substrate in AMP buffer at pH 9.4.

    Main Results:

    • A linear relationship was observed between reciprocal enzyme activity and theophylline concentration (2-60 mg/L).
    • Minimal interference from 3-methylxanthine (≤1 mg/L apparent increase).
    • High correlation with immunoenzymic assay results, demonstrating accuracy and specificity.

    Conclusions:

    • The described method provides a simple, accurate, and cost-effective means for serum theophylline quantification.
    • Reagent stability and instrumentation simplicity are key advantages for clinical application.
    • The assay is robust against common interfering substances and drug interactions.

    Related Experiment Videos