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In vivo effect of UV-B on lymphocyte function
The British Journal of Dermatology
|November 1, 1979
Summary
UV-B radiation exposure temporarily alters immune cell populations in normal subjects. Specifically, it reduces E-rosette-forming lymphocytes and increases null cells, impacting DNA synthesis in lymphocytes.
Area of Science:
- Immunology
- Photobiology
- Dermatology
Background:
- Ultraviolet-B (UV-B) radiation is known to have immunosuppressive effects.
- Understanding the specific impact of UV-B on lymphocyte subsets and function is crucial for assessing health risks.
Purpose of the Study:
- To investigate the effects of whole-body erythemogenic UV-B radiation on circulating lymphocyte populations and DNA synthesis in normal subjects.
- To determine the dose-dependency and time course of these UV-B-induced immunological changes.
Main Methods:
- Normal subjects were exposed to erythemogenic doses of UV-B radiation.
- Circulating lymphocytes were analyzed for E-rosette-forming cells and null cells.
- Lymphocyte DNA synthesis was measured by tritiated thymidine incorporation after phytohaemagglutinin (PHA) stimulation.
Main Results:
- UV-B exposure led to a decreased proportion of E-rosette-forming lymphocytes.
- An increased proportion of null cells was observed post-exposure.
- A reduced incorporation of tritiated thymidine into lymphocyte DNA following PHA stimulation was noted.
- These changes were dose-dependent, appearing rapidly, peaking at 8-12 hours, and resolving within 48-72 hours.
Conclusions:
- Whole-body UV-B radiation exposure induces significant, transient alterations in lymphocyte populations and function.
- The observed immunosuppressive effects are dose-dependent and reversible, highlighting the dynamic nature of UV-B radiation's impact on the immune system.