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IgA in commercial gamma-globulin preparations.
Scandinavian Journal of Immunology
|January 1, 1976
Summary
Adverse reactions may occur from immunoglobulin A (IgA) in gamma-globulin injections. This study found altered IgA forms in preparations, potentially increasing immunization risks.
Area of Science:
- Immunology
- Biochemistry
- Pharmacology
Background:
- Anti-immunoglobulin A (IgA) antibodies are linked to adverse reactions from IgA-containing materials.
- Gamma-globulin preparations are commonly used for therapeutic purposes.
Purpose of the Study:
- To investigate the presence and forms of IgA in commercial gamma-globulin preparations.
- To assess the potential risk of IgA immunization associated with these preparations.
Main Methods:
- Immuno-gel filtration was used to analyze IgA components.
- Immunoelectrophoresis-immunofluorescence detected IgG-IgA complexes.
- Reduction-alkylation was employed to degrade IgA aggregates.
Main Results:
- IgA was detected in all investigated gamma-globulin preparations, though levels varied.
- Analysis revealed IgA aggregates, a 7S component, and fragments.
- IgG-IgA complexes were identified, and IgA aggregates were largely removed by reduction-alkylation.
Conclusions:
- Altered forms of IgA, including aggregates and complexes, are present in gamma-globulin preparations.
- These modified IgA forms may elevate the risk of developing anti-IgA antibodies after injection.