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Related Experiment Videos

Microscopically and chemically detected haemolacria.

M S Norn

    Acta Ophthalmologica
    |February 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Occult blood in tears (haemolacria) can be detected using microscopy or chemical tests. Microscopy is more sensitive, identifying blood in 13% of normal eyes, while chemical tests detect it in 3%.

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    Area of Science:

    • Ophthalmology
    • Clinical Chemistry

    Background:

    • Occult haemolacria, or hidden blood in tears, is a condition requiring sensitive detection methods.
    • Understanding the prevalence and causes of haemolacria is crucial for diagnosing and managing various ocular conditions.

    Purpose of the Study:

    • To develop and compare two methods for detecting occult blood in tears: microscopy and chemical stix tests.
    • To determine the prevalence of occult haemolacria in normal individuals and in patients with different types of conjunctivitis.

    Main Methods:

    • Microscopy of conjunctival fluid using a quantitative pipette method.
    • Chemical stix testing on conjunctival fluid samples transferred to a cotton-wool plug.
    • Analysis of samples from 306 eyes for microscopy and 303 eyes for chemical tests.

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    Main Results:

    • Microscopy detected occult blood in 13% of normal eyes, compared to 3% with chemical tests.
    • Occult blood was most prevalent in acute infectious conjunctivitis (65% microscopically, 21% chemically).
    • Haemolacria correlated with neutrophilia (45%) and lymphocytosis (35%), and tear protein concentration was elevated.

    Conclusions:

    • Microscopy is a more sensitive method for detecting occult haemolacria than chemical stix tests.
    • Occult haemolacria is frequently associated with infectious conjunctivitis and certain white blood cell patterns.
    • The presence of blood in tears postoperatively is transient, while instilled blood clears rapidly.