Effects of caffeine on mitotic index, mitotic aberrations and bimitosis with and without aeration

The effects of 1 to 3 h 0.2% caffeine treatment on mitosis in lateral roots of Vicia faba with and without aeration have been investigated. During the treatment a marked decrease of the mitotic index followed by strong deviations and changing phase indices can be stated. By means of aeration the number of mitotic aberrations increases with time of treatment, while it decreases without aeration until 3 h treatment. Tetraploid cells are supposed to be formed by spindle aberrations at early anaphase. The number of binucleate and tetraploid cells is affected by aeration during caffeine treatment. During division of the binucleate cells tetraploid nuclei are formed by fusions, so the population of binucleate cells may become smaller.