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Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms
Abstract:
Contamination of Strain L cell cultures by pleuropneumonia-like organismtis (PPLO) resulted in a complete inhibition of the incorporation of tritiated thymnidine and uridine. Contanminated cultures were characterized by a concentration of PPLO along the margins of the cells and in the intercellular-spaces of the cultures. Autoracliograins of PPLO-contaminated cultures were characterized by exposed silver grains over the margins of the cells. Kanamnycin was efjective in the elimination of PPLO and the restoration of nucleoside incorporation.
Insights
Pleuropneumonia-like organisms (PPLO) contaminated cell cultures, inhibiting nucleic acid synthesis. Kanamycin effectively eliminated PPLO, restoring normal cell function and nucleoside incorporation.
Area of Science:
- Cell culture contamination
- Microbiology
- Molecular biology
Background:
- Strain L cell cultures are susceptible to microbial contamination.
- Pleuropneumonia-like organisms (PPLO) can interfere with cellular processes.
Purpose of the Study:
- To investigate the impact of PPLO contamination on nucleoside incorporation in cell cultures.
- To evaluate the efficacy of kanamycin in treating PPLO contamination.
Main Methods:
- Strain L cells were cultured and intentionally contaminated with PPLO.
- Tritiated thymidine and uridine incorporation assays were performed.
- Autoradiography was used to visualize PPLO localization.
- Kanamycin treatment was administered to contaminated cultures.
Main Results:
- PPLO contamination completely inhibited tritiated thymidine and uridine incorporation.
- PPLO were observed at the cell margins and intercellular spaces.
- Autoradiography confirmed PPLO presence at cell margins.
- Kanamycin treatment eliminated PPLO and restored nucleoside incorporation.
Conclusions:
- PPLO contamination significantly disrupts nucleic acid synthesis in cell cultures.
- Kanamycin is an effective treatment for PPLO contamination in cell cultures.
- Restoration of nucleoside incorporation indicates successful PPLO eradication.