[Pharmacokinetic analysis of a benzarone preparation. First communication: analytical method (author's transl)]

A new analytical method to determine 3-(4-hydroxybenzoyl)-2-ethyl-benzofurane (benzarone, Fragivix) from human serum and urine is described. The substance is extracted from the biological fluids with chloroform after cleavage of the glucuronide with glucuronidase. Benzarone is detected with the HPLC with a special design method on the base of a gradient elution on a reversed-phase column. The method is suitable to measure the necessary concentrations for a kinetic analysis in the serum as well as in the urine. The limit of assessment could be detected at 0.03 microgram/ml. The serum concentration-time curve after oral as well as after i.v. application could be detected with the method.