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Relation between common antigen and membrane antigens associated with Marek's disease herpesvirus and turkey
Abstract:
The appearance of two kinds of membrane antigen (MA) in Marek's disease herpesvirus (MDHV) or herpesvirus of turkey (HVT) infected cells was examined using antiserum to the common antigen (common-Ag). No early-appearing MA(EMA) was detected in these cultures until 16 hours post-inoculation, after which a number of cells in these cultures had late-appearing membrane antigen (LMA) as detected by immunofluorescence (IF). Fluids from cultures infected with these viruses were examined for the presence of the common-Ag by agar-gel precipitation (AGP) test. No common-Ag was detected until 16 hours, but after 24 hours, it was detected by AGP test. These results suggest that the common-Ag is related to LMA but not to EMA.
Insights
Researchers investigated membrane antigens in Marek's disease herpesvirus (MDHV) and turkey herpesvirus (HVT) infections. The common antigen (common-Ag) appeared later and correlated with late-appearing membrane antigen (LMA), not early-appearing membrane antigen (EMA).
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Marek's disease herpesvirus (MDHV) and herpesvirus of turkey (HVT) are significant avian pathogens.
- Understanding viral antigen expression is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the temporal expression of membrane antigens (MA) in MDHV and HVT infected cells.
- To determine the relationship between early-appearing membrane antigen (EMA), late-appearing membrane antigen (LMA), and common antigen (common-Ag).
Main Methods:
- Immunofluorescence (IF) was used to detect EMA and LMA in infected cell cultures.
- Agar-gel precipitation (AGP) test was employed to detect common-Ag in culture fluids.
- Antiserum to common-Ag was utilized for detection.
Main Results:
- EMA was not detected until 16 hours post-inoculation.
- LMA was observed in a subset of infected cells after 16 hours.
- Common-Ag was detected in culture fluids by AGP test after 24 hours post-inoculation.
- No common-Ag was detected before 16 hours.
Conclusions:
- The common antigen (common-Ag) is associated with late-appearing membrane antigen (LMA).
- The common antigen (common-Ag) is not related to early-appearing membrane antigen (EMA).
- These findings contribute to understanding the antigen dynamics during MDHV and HVT infections.