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Abstract:
Werly, Emil F. (Midwest Research Institute, Kansas City, Mo.), and Anne Monley. Assay of T(3) phage by plaque count. J. Bacteriol. 87:1177-1179. 1964.-T(3) phage-count determinations were made by the agar layer method, with various media being used for serial dilutions, plate media, and overlayer media. Plaques produced with Tryptose-phosphate-dextrose hard agar as plate and overlayer media were small, distinct, sharply defined, and easily counted. With the media described, several hundred plaques per plate can be counted, after either 4 or 20 hr of incubation.
Insights
This study details a plaque count method for assaying T(3) phage. Tryptose-phosphate-dextrose agar enables easy counting of several hundred T(3) phage plaques within 20 hours.
Area of Science:
- Microbiology
- Virology
- Bacteriology
Background:
- Accurate quantification of bacteriophages is crucial for various research applications.
- Traditional plaque counting methods can be time-consuming and may lack precision.
Purpose of the Study:
- To establish an efficient and reliable method for the assay of T(3) phage using plaque counts.
- To optimize media conditions for clear and countable plaque formation.
Main Methods:
- The agar layer method was employed for bacteriophage T(3) enumeration.
- Serial dilutions were performed using various media.
- Tryptose-phosphate-dextrose hard agar was utilized as both plate and overlayer media.
Main Results:
- Plaques generated with Tryptose-phosphate-dextrose agar were consistently small, distinct, and sharply defined.
- This method allowed for the easy counting of several hundred plaques per plate.
- Effective plaque counting was achievable after incubation periods of both 4 and 20 hours.
Conclusions:
- The described agar layer method using Tryptose-phosphate-dextrose agar provides a robust technique for T(3) phage titration.
- This optimized method enhances the accuracy and efficiency of bacteriophage quantification.