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High affinity estrogen binding by rabbit liver.

B J Danzo, V Krishnamurthy, B C Eller

    Biochimica Et Biophysica Acta
    |December 22, 1977
    PubMed
    Summary
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    Rabbit liver cytosol contains two [3H]estradiol binding components. A specific, high-affinity estrogen binder (8-9 S) and a non-specific binder (4-5 S) were identified, differing in steroid specificity and physicochemical properties.

    Area of Science:

    • Biochemistry
    • Endocrinology
    • Molecular Biology

    Background:

    • Macromolecular binding components for [3H]estradiol-17beta exist in rabbit liver cytosol.
    • Previous studies suggest the presence of estrogen binders in various tissues.

    Purpose of the Study:

    • To characterize the [3H]estradiol binding components in rabbit liver cytosol.
    • To differentiate between specific and non-specific binding of estradiol.

    Main Methods:

    • Incubation of rabbit liver cytosol with [3H]estradiol.
    • Analysis of binding activity using low and high ionic strength sucrose gradients.
    • Steroid specificity and physicochemical parameter assessment.

    Main Results:

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  • Two binding components detected: a 4-5 S component and an 8-9 S component.
  • The 8-9 S component exhibited estrogen-specific, saturable, high-affinity binding (Kd = 3.05 x 10(-10) M).
  • The 4-5 S component showed non-saturable binding, not inhibited by other steroids.

    • Conclusions:

    • Rabbit liver cytosol contains at least two distinct estradiol binding components.
    • A high-affinity estrogen receptor is present, along with a non-specific binding moiety.
    • Estrogen binders are found in liver cytosol across different rabbit ages and sexes.